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Anti -Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin)
Anti -Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Ficolin-2 is a lectin that contains fibrinogen-like and collagen-like domains. It circulates as a tetramer of disulfide-linked homotrimers. Ficolin-2 associates with MASP proteins to activate complement. | | | Catalog # | F4499-50H | | Hybridoma | Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Ficolin-2 (rhFicolin-2). | | Applications | Western Blot, Direct ELISA | | Recommended Dilution | Western Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human Ficolin-2. Using a colorimetric detection system, the detection limit for rhFicolin-2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. | | Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human Ficolin-2. The detection limit for rhFicolin-2 is approximately 1 ng/well. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 8H139 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | NS0-derived rhFicolin-2 | | Specificity | Recognizes human Ficolin-2. Does not crossreact with rhFicolin-3. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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