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You are here:Home » Antibodies » Abs to Lectins » Anti -Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin)

Anti -Ficolin 2 (Ficolin-B, Ficolin beta, L-ficolin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Ficolin-2 is a lectin that contains fibrinogen-like and collagen-like domains. It circulates as a tetramer of disulfide-linked homotrimers. Ficolin-2 associates with MASP proteins to activate complement.
Catalog #F4499-50H
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Ficolin-2 (rhFicolin-2).
ApplicationsWestern Blot, Direct ELISA
Recommended DilutionWestern Blot: This antibody can be used at 1-2ug/mL with the appropriate secondary reagents to detect human Ficolin-2. Using a colorimetric detection system, the detection limit for rhFicolin-2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Direct ELISA: This antibody can be used at 0.5-1.0ug/mL with the appropriate secondary reagents to detect human Ficolin-2. The detection limit for rhFicolin-2 is approximately 1 ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H139
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS, 5% trehalose. Reconstitution: Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rhFicolin-2
SpecificityRecognizes human Ficolin-2. Does not crossreact with rhFicolin-3.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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