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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Filamin

Anti -Filamin


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Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH IF
Filamin functions as a crosslinking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts and smooth muscle actin.
Catalog #F4510-05
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:250-1:1000
Immunoprecipitation: Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5ul of antibody for
every 300-500ul of cell lysate (200-500ug/ml total protein is suggested. Incubation is 1hour at RT or overnight at 4ºC; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-10% acrylamide gels are recommended for full length filamin or the 80kD fragment visualization.
Immunofluorescence (ABC):1:50-1:200
Immunohistochemistry (paraffin): (lower dilutions) High temperature citrate buffer antigen retrieval technique recommended.
Optimal dilution determined by the researcher.
Recommended Positive Control TissueHuman or bovine skin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No2Q1092
ConcentrationNot determined
FormSupplied as a liquid. No preservatives added.
ImmunogenHuman platelet protein
SpecificityRecognizes unprocessed, full length human filamin (actin-binding protien, 270-280kD) as well as the 90kD C-term calpain cleavage fragment of filamin. Species Crossreactivity: bovine. Does not react with mouse or rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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