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You are here:Home » Antibodies » Abs to Binding Proteins » Anti -FBLIM1 (FBLP1, Filamin-binding LIM Protein 1, Migfilin, Mitogen-inducible 2-interacting Protein, MIG2-interacting Protein, CAL, DKFZp434G171, FBLP-1, RP11-169K16.5)

Anti -FBLIM1 (FBLP1, Filamin-binding LIM Protein 1, Migfilin, Mitogen-inducible
2-interacting Protein, MIG2-interacting Protein, CAL, DKFZp434G171, FBLP-1, RP11-169K16.5)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Migfilin is a recently identified protein involved in linking the plasma membrane to the actin cytoskeleton as a component of cell-cell and cell-ECM adhesions through the binding of filamin proteins and plays a role in the restructuring of the actin cytoskeleton and regulation of cell shape. A widely expressed protein, migfilin is composed of a filamin binding domain found in the N-terminus, a central proline-rich domain, and three LIM domains within the C-terminus. These LIM domains assist in kindlin-2 binding and are necessary components for localization to focal adhesions. Recent studies show that the central proline-rich domain binds to vasodilator-stimulated phosphoproteins, which seem to be a requirement of migfilinís ability to affect cell migration. The N-terminal domainís role in binding filamin is what allows migfilin to link with the actin cytoskeleton. Removal of the final LIM domain and the central proline-rich domain results in alternative splicing, and three isoforms of migfilin have been observed. All isoforms are shown to be expressed in kidney, pancreas, lung, placenta, heart and platelets. Isoform 2 has also been observed in brain and skeletal muscle tissue. Levels of migfilin considered to be higher than the normal levels of expression have been associated with human leiomyosarcoma.
Catalog #F4510-91
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1k
Clone No10H14
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protien G affinity chromatography.
ImmunogenRecombinant protein corresponding to human Migfilin (GST-tagged).
SpecificityRecognizes human Migfilin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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