Anti -Filamin-binding LIM Protein 1 (FBLIM1, FBLP1, FBLP-1, CAL, DKFZp434G171, Migfilin,
Mitogen-inducible 2-interacting Protein, MIG2-interacting Protein, RP11-169K16.5)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B |
|
| Migfilin is a recently identified protein involved in linking the plasma membrane to the actin cytoskeleton as a component of cell-cell and cell-ECM adhesions through the binding of filamin proteins and plays a role in the restructuring of the actin cytoskeleton and regulation of cell shape. A widely expressed protein, migfilin is composed of a filamin binding domain found in the N-terminus, a central proline-rich domain, and three LIM domains within the C-terminus. These LIM domains assist in kindlin-2 binding and are necessary components for localization to focal adhesions. Recent studies show that the central proline-rich domain binds to vasodilator-stimulated phosphoproteins, which seem to be a requirement of migfilin’s ability to affect cell migration. The N-terminal domain’s role in binding filamin is what allows migfilin to link with the actin cytoskeleton. Removal of the final LIM domain and the central proline-rich domain results in alternative splicing, and three isoforms of migfilin have been observed. All isoforms are shown to be expressed in kidney, pancreas, lung, placenta, heart and platelets. Isoform 2 has also been observed in brain and skeletal muscle tissue. Levels of migfilin considered to be higher than the normal levels of expression have been associated with human leiomyosarcoma. | | | Catalog # | F4510-91 | | Applications | | | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 0.5ug/ml | | Optimal dilutions to be determined by the researcher. | | Positive Control | HeLa cell lysate | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1k | | Clone No | 10H14 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide. | | Purity | Purified by Protien G affinity chromatography. | | Immunogen | Recombinant protein corresponding to human Migfilin (GST-tagged). | | Specificity | Recognizes human Migfilin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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