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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B FC IF

The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
Catalog #	F4524-72A
Applications	Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended Dilutions	ELISA: 0.01-0.05ug/ml
	Western Blot: 0.1-1.0ug/ml
	Flow Cytometry: 1-3ug Labels 1x10e6 cells
	Immunofluorescence: 5-20ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to DYKDDDDK Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, conjugated to KLH.
Specificity	Recognzies DYKDDDDK tagged fusion proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.