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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Flag (DYKDDDDK) Epitope Tag (Alexa FluorŪ488)

Anti -Flag (DYKDDDDK) Epitope Tag (Alexa FluorŪ488)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified FC IF
The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
Catalog #F4524-72C
ApplicationsSuitable for use in Flow Cytometry and Immunofluorescence. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50
Immunofluorescence (IF-IC) 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing Alexa Fluor® conjugates will result in a substantial loss of activity. Alexa Fluor® conjugates are sensitive to light.
Clone TypePolyclonal
HostRabbit
ConcentrationNot determined
FormSupplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.1% sodium azide. Labeled with Alexa Fluor®488.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenSynthetic peptide corresponding to DYKDDDDK conjugated to KLH.
SpecificityRecognizes exogenously expressed DYKDDDDK proteins in cells. Recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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