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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Flag (DYKDDDDK) Epitope Tag

Anti -Flag (DYKDDDDK) Epitope Tag

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #F4524-72F
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1:50,000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSuppied as liquid in PBS, pH 7.2, 0.01% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenDYKDDDDK epitope tag peptide DYKDDDDK, conjugated to KLH.
SpecificityRecognizes the DYKDDDDK epitope tag fused to the N-terminus of targeted proteins. Does not react with fusion proteins containing the DYKDDDDK epitope tag at the C-terminus. In Western Blot against bacterial extracts, it does not crossreact with endogenous proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)


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