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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Catalog #	F4524-72F
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	ELISA: 1:10,000-1:50,000
	Western Blot: 1:1000-1:5000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~1mg/ml
Form	Suppied as liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	DYKDDDDK epitope tag peptide DYKDDDDK, conjugated to KLH.
Specificity	Recognizes the DYKDDDDK epitope tag fused to the N-terminus of targeted proteins. Does not react with fusion proteins containing the DYKDDDDK epitope tag at the C-terminus. In Western Blot against bacterial extracts, it does not crossreact with endogenous proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.