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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Flag (DYKDDDDK) Epitope Tag (HRP)

Anti -Flag (DYKDDDDK) Epitope Tag (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #F4524-72H
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:10,000-1:50,000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 0.01% gentamicin sulfate 1% BSA. Labeled with horseradish peroxidase (HRP). Reconstitute with 100ul sterile ddH2O.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to Enterokinase cleavage site, DYKDDDDK (KLH). Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.
SpecificityRecognizes DYKDDDDK when fused to either the amino or carboxyl terminus of target proteins. Shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence. Does not crossreact with any endogenous proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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