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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Flag (DYKDDDDK) Epitope Tag

Anti -Flag (DYKDDDDK) Epitope Tag

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified E B
The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
Catalog #F4524-72L
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.2-1.0ug/ml
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken-HRP Rb x Ch
I1906-12: IgY, Chicken-FITC Rb x Ch
I1906-14: IgY, Chicken-AP Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgY
HostChicken
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenFlag (DYKDDDDK) Epitope Tag, conjugated to KLH.
SpecificityRecognizes Flag Epitope Tag.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)


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