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You are here:Home » Antibodies » Antibodies-Epitope Tags » Anti -Flag (DYKDDDDK) Epitope Tag (HRP)

Anti -Flag (DYKDDDDK) Epitope Tag (HRP)


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Clone Host Grade Applications
Polyclonal Chicken Affinity Purified E B
Catalog #F4524-72P
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:15,000
Western Blot: 1:500-1:2500
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906-01: IgY, Fc (HRP) Pab Gt x Ch
I1906-10: IgY (HRP) Pab Rb x Ch
I1906-12: IgY (FITC) Pab Rb x Ch
I1906-14: IgY (AP) Pab Rb x Ch
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in 25mM HEPES, pH 7.0, 0.05M sodium chloride, 0.1% BSA, 0.005% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
PurityPurified by immunoaffinity chromatography.
ImmunogenFlag Epitope (DYKDDDDK) conjugated to KLH.
SpecificityRecognizes Flag Epitope Tag.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)

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