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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Flag (DYKDDDDK) Epitope Tag

Anti -Flag (DYKDDDDK) Epitope Tag

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC IF
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteinís biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors.
Catalog #F4524-72W
ApplicationsSuitable for use in Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9F166
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4 15mM sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to DYKDDDK, conjugated KLH.
SpecificityRecognizes Flag (DYKDDDDK) epitope tag. Recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decreases the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)


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