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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Flag (DYKDDDDK) Epitope Tag

Anti -Flag (DYKDDDDK) Epitope Tag

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors.
Catalog #F4524-72X
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No12K259
HostMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder from 0.01M PBS, pH 7.2. Reconstitute in 500ul sterile ddH2O.
PurityPurified by size exclusion.
ImmunogenSynthetic peptide DYKDDDDK (KLH).
SpecificityRecognizes DYKDDDDK-tagged recombinant proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)


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