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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Flag (DYKDDDDK) Epitope Tag

Anti -Flag (DYKDDDDK) Epitope Tag

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain DNA vectors containing the particular tagged oligo fragments. These short oligo fragments encode small peptides (6xHis, c-Myc, HA, DYKDDDDK) or small proteins (GST, MBP, beta-Galactosidase). The antibodies against these fusion-tags can be used to monitor and purify the fusion protein.
Catalog #F4524-72Y
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the sequence DYKDDDDK.
SpecificityRecognizes DYKDDDDK Tag in all species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)


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