Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution
Western Blot: 0.5-2ug/ml detects Flk 1 in RIPA lysates from HUVEC cells. HUVEC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F4565-05 (2ug/ml). Proteins were visualized using a *goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1158-1345 of mouse Flk 1.
Form
Supplied as a liquid in 20mM sodium phosphate, pH 7.6, 250mM sodium chloride, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse Flk 1, Mr ~230kD and an immature form of the protein, Mr ~200kD. Species Crossreactivity: human
References
1. Thuringer, D. et al., J. Biol. Chem. 277:2028-2032 (2002).