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Abs to Receptors, Tyrosine Kinase
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Anti -Flk 1, aa1158-1345 (KDR, VEGF Receptor, Receptor Tyrosine Kinase)
Anti -Flk 1, aa1158-1345 (KDR, VEGF Receptor, Receptor Tyrosine Kinase)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Catalog # | F4565-05 | | Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot Analysis: 0.5-2ug/ml detects KDR/Flk-1 in RIPA lysates from HUVEC cells. HUVEC cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F4565-05 (2ug/ml). Proteins were visualized using a *goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | *Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 3B6 | | Host | Mouse | | Source | Mouse | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM sodium phosphate, pH 7.6, 250mM sodium chloride, 0.1% sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein corresponding to aa1158-1345 of mouse KDR/Flk-1. | | Specificity | Recognizes mouse KDR/Flk-1, Mr ~230kD and an immature form of the protein, Mr ~200kD. Species Crossreactivity: human | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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