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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2)

Anti -Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Flk-1/KDR/vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
Catalog #F4565-08
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 5-10ug/ml for 2hrs at RT.
Immunohistochemistry (formalin/paraffin): 1:50 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Positive ControlHey cells. Angiosarcoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See F4565-08X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide from C-terminus of the precursor form of the mouse Flk-1. Cellular Localization: Cytoplasmic
SpecificityRecognizes Flk-1 (KDR , VEGFR2). Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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