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Anti -Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2) (BSA & Azide Free)
Anti -Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2) (BSA & Azide Free)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH |
|
| Flk-1/KDR/vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4. | | | Catalog # | F4565-08X | | Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Western Blot: 5-10ug/ml for 2hrs at RT. Detects band at ~180kD. | | Immunohistochemistry (formalin/paraffin): 1:25 for 60 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. | | Optimal dilution determined by the researcher. | | Positive Control | Hey cells. Angiosarcoma | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%. Also available with BSA and azide. See F4565-08. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide from C-terminus of the precursor form of the mouse Flk-1 (~180kD). Cellular Localization: Cytoplasmic. | | Specificity | Recognizes Flk-1 (KDR , VEGFR2). Species Crossreactivity: Human, mouse and rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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