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You are here:Home » Antibodies » Abs to Receptors, Tyrosine Kinase » Anti -Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2) (BSA & Azide Free)

Anti -Flk-1 Receptor Tyrosine Kinase (KDR, VEGFR2) (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
Flk-1/KDR/vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4.
Catalog #F4565-08X
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 5-10ug/ml for 2hrs at RT. Detects band at ~180kD.
Immunohistochemistry (formalin/paraffin): 1:25 for 60 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Positive ControlHey cells. Angiosarcoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%. Also available with BSA and azide. See F4565-08.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide from C-terminus of the precursor form of the mouse Flk-1 (~180kD). Cellular Localization: Cytoplasmic.
SpecificityRecognizes Flk-1 (KDR , VEGFR2). Species Crossreactivity: Human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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