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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.
Catalog #	F4568-01B
Applications	Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
	Peptide ELISA Titer: 1:32,000
	Western Blot: 0.3-1ug/ml ~48kD band observed in lysates of cell line H460 (calculated MW of 47.4kD according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Purity	Purified by peptide affinity chromatography.
Immunogen	Synthetic peptide corresponding to C-SISQVNHKPLRTA, from the C Terminus of human Flotillin 1 (NP_005794.1).
Specificity	Recognizes human Flotillin 1. Species sequence homology: canine, porcine, bovine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	DADB-118P11.2, integral membrane component of caveolae, reggie-2, flotillin 1, FLOT1