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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -FLRT3 (Fibronectin Leucine-Rich Transmembrane Protein 3)

Anti -FLRT3 (Fibronectin Leucine-Rich Transmembrane Protein 3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Human Fibronectin Leucine-rich Transmembrane protein 3 (FLRT3) is a 95kD type I transmembrane (TM) glycoprotein that belongs to a small and unique family of leucine-rich repeat proteins. It is synthesized as a 649 amino acid (aa) precursor that contains a 28aa signal sequence, a 500aa extracellular domain (ECD), a 21aa TM segment and a 100aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats which are flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain. The molecule reportedly has neurite outgrowth promoting activity. The human FLRT3 ECD shares 96% aa sequence identity with mouse FLRT3 ECD.
Catalog #F4598-40
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.
Direct ELISA: 0.5-1.0ug/ml. The detection limit for rhFLRT3 is ~3ng/well.
Optimal dilutions should be determined by each laboratory for each application.
HybridomaFusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human FLRT3 (rhFLRT3; aa29-528: Accession # AAH20870).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG3
Clone No7H131
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography
ImmunogenNS0-derived rhFLRT3
SpecificityRecognizes human FLRT3. Does not crossreact with human FLRT1 or FLRT2. Species Sequence Homology: mouse, 96%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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