| Flt1 (human 1338aa, rat 1336aa, mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt1 is a member of the flt family of receptor tyrosine kinases that also includes KDR/flk-1 and flt4. The flt family encodes receptors for VEGF that are characterized by seven Ig-like loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. It has been reported that flt1 receptor dimerization precedes phosphorylation after VEGF binding. Flt1 has also been described as VEGFR1 and Flk2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart and brain but not in tumor cells. |
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| Catalog # | F4599 |
| Applications | Suitable for use in Western Blot. Not suitable for use in Immunoprecipitation or Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1ug/ml detected recombinant Flt1 in a membrane preparation from Hi5 insect cells expressing recombinant Flt1. Hi5 insect cells expressing recombinant Flt1 membrane prep cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with 1ug/ml of F4599. Proteins were visualized using an IgG (HRP) goat anti-chicken and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1906: IgY, Chicken Pab Rb x Ch |
| I1906-10: IgY, Chicken (HRP) Rb x Ch |
| I1906-12: IgY, Chicken (FITC) Rb x Ch |
| I1906-14: IgY, Chicken (AP) Rb x Ch |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgY |
| Host | Chicken |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a solution in sterile PBS, 0.05% sodium azide. |
| Purity | IgY purified by PEG and ammonium sulfate precipitation. |
| Immunogen | Synthetic peptide corresponding to aa1209-1230, DDVRYVNAFKFMSLERIKTFEE of human Flt1 (KLH). |
| Specificity | Recognizes human Flt1 at ~180kD. Species Sequence Homology: Mouse, rat |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
