| Flt-1 (human 1338aa, rat 1336aa and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt-1 (fms-like tyrosine kinase-1) is a member of the flt family of receptor tyrosine kinases that also includes KDR/flk-1 and flt-4. The flt family encodes receptors for VEGF (vascular endothelial growth factor) that are characterized by seven Ig-like loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. It has been reported that flt-1 receptor dimerization precedes phosphorylation after VEGF binding. Flt-1 has also been described as VEGFR1 and Flk-2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells. |
|
| Catalog # | F4599-03B |
| Applications | Suitable for use in Western Blot. Not recommended for Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Western Blot: 0.5-2ug/ml Detected Flt1 in human umbilical vein endothelium (HUVEC) membrane preps. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 5H17 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | GST fusion protein corresponding to aa1251-1338 of human Flt1. |
| Specificity | Recognizes human Flt1, Mr ~180kD. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
