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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Flt3 Ligand (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)

Anti -Flt3 Ligand (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Flt3-ligand (flk2-ligand), which stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells, is a soluble 17.6kD polypeptide containing 156 amino acid residues comprising the extracellular domain of the transmembrane flt3-ligand protein.
Catalog #F4600-07
ApplicationsSuitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended DilutionNeutralization: 0.25-0.75ug/ml to yield one-half maximal inhibition [ND50] of the biological activity (15ng/ml).
ELISA: 0.5ug/ml. Direct ELISA (using 100ul/well antibody solution). This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder from 0.5X PBS, pH 7.2. Reconstitute with sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified recombinant human Flt3-Ligand
SpecificityRecognizes human Flt3-Ligand.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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