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Anti -Flt3 Ligand (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)
Anti -Flt3 Ligand (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B N |
|
| Flt3-ligand (flk2-ligand), which stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells, is a soluble 17.6kD polypeptide containing 156 amino acid residues comprising the extracellular domain of the transmembrane flt3-ligand protein. | | | Catalog # | F4600-07 | | Applications | Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested. | | Recommended Dilution | Neutralization: 0.25-0.75ug/ml to yield one-half maximal inhibition [ND50] of the biological activity (15ng/ml). | | ELISA: 0.5ug/ml. Direct ELISA (using 100ul/well antibody solution). This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well. | | Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from 0.5X PBS, pH 7.2. Reconstitute with sterile 40-50% glycerol, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Highly purified recombinant human Flt3-Ligand | | Specificity | Recognizes human Flt3-Ligand. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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