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You are here:Home » Antibodies » Antibodies-Tyrosine Kinases » Anti -Flt3 Ligand (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)

Anti -Flt3 Ligand (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B N
Flt3-ligand (flk2-ligand), which stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells, is a soluble 17.6kD polypeptide containing 156 amino acid residues comprising the extracellular domain of the transmembrane flt3-ligand protein.
Catalog #F4600-07
ApplicationsSuitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended DilutionNeutralization: 0.25-0.75ug/ml to yield one-half maximal inhibition [ND50] of the biological activity (15ng/ml).
ELISA: 0.5ug/ml. Direct ELISA (using 100ul/well antibody solution). This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder from 0.5X PBS, pH 7.2. Reconstitute with sterile 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly purified recombinant human Flt3-Ligand
SpecificityRecognizes human Flt3-Ligand.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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