Anti -Flt3 (Fms-like Tyrosine Kinase 3, FLT-3, CD135, FL Cytokine Receptor, FLK2,
Fms-related Tyrosine Kinase 3, Stem Cell Tyrosine Kinase 1, STK1, STK-1, Tyrosine-protein
Kinase Receptor FLT3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
E B IP IH |
|
| FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation . Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia. | | | Catalog # | F4600-07B | | Applications | Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications not tested. | | Recommended Dilutions | Immunoprecipiation: 1:100 | | Immunohistochemistry (Paraffin): 1:100 | | Western Blot: 1:1000; Bands at 130 and 160kD. 130kD - Non-glycosylated form; 160kD - Mature, glycosylated form. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 7C1 | | Host | Rabbit | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide. | | Purity | Supernatant | | Immunogen | Synthetic peptide corresponding to the sequence surrounding Ser740 of human FLT3 (KLH). | | Specificity | Recognizes human Flt3 Ligand. Detects endogenous levels of FLT3 protein. Does not crossreact with related proteins. | | Species Crossreactivity | Mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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