| Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in vitro in cooperation with other cytokines. Flt3 is also expressed in the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. |
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| Catalog # | F4600-08 |
| Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 0.5-2ug/ml detected Flt3 in RIPA lysates from HeLa cells and mouse Bafl-3 cells. Bafl-3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with F4600-08 (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunoprecipitation: 4ug immunoprecipitated Flt3 from 500ug of mouse Bafl-3 RIPA lysate. |
| Immunocytochemistry: 10ug/ml showed positive immunostaining for Flt3 in mouse Bafl-3 cells fixed with methanol and permeabilized with 0.25% Triton X-100 for 5 mins. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | Western Blot: |
| I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Immunocytochemistry: |
| I1904-40D: IgG, H&L (FITC) Pab Gt xRb |
| I1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Mouse |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | His-tag fusion protein corresponding to aa786-1000 of mouse Flt3. |
| Specificity | Recognizes the processed (130kD) and the mature forms (155kD) of mouse Flt3. Species Crossreactivity: human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
