| FMS-related tyrosine kinase 3 (FLT3, also called Flk2), a member of the type III receptor tyrosine kinase family that includes c-Kit, PDGF and M-CSF receptors, is expressed on early hematopoietic progenitor cells, and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream cellular targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4–6). Tyrosine 591 is located in the juxtamembrane region of FLT3. It may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7). |
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| Catalog # | F4600A |
| Application(s) | Suitable for use in ELISA, Western Blot. |
| Recommended Dilution | ELISA: 100ul/well antibody detects hFLT3-ligand by direct ELISA. Use at a concentration of 0.15-0.30ug/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFLT3-ligand. |
| Western Blot | To detect hFLT3-ligand by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFLT3-ligand is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. |
| Biological Activity | A 1:500 dilution of a 1 mg/ml antibody solution will give satisfactory results in an ELISA when 1ug/well human flt3-ligand is used to coat the plate. |
| Reconstitution | The biotinylated antibody should be reconstituted to a concentration of 50ug/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4°C for at least one month without detectable loss of activity. |
| Stability | The lyophilized antibody is stable for at least 6 months when kept at -20°C. |
| Country of Origin | USA |
| CAS Number | n/a |
| Clone Type | |
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| Elisa |
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| To detect hFLT3-ligand by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFLT3-ligand. |
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| Western Blot |
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| To detect hFLT3-ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFLT3-ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
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| Country of Origin | |
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| USA |
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| Size A |
| 25 µg |
| $170 US |
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| Size B |
| 50 µg |
| $240 US |
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| Special Sizes | |
| Many products can be ordered in custom sizes. Please click here to contact Peprotech with your request. |
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| Order Online from our two international web sites | |
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| PeproTech Inc. Cytokines for the Americas |
| Princeton Business Park • 5 Crescent Ave. • P.O. Box 275 • Rocky Hill, NJ 08553 |
| Tel | (800) 436-9910 • (609) 497-0253 • Fax: (609) 497-0321 |
| URL | Www.peprotech.com •E-mail: info@peprotech.com •sales@peprotech.com |
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| PeproTech EC Ltd. Cytokines for Europe, Japan, and Asia |
| PeproTech House • 29 Margravine Road • London W6 8LL • UK |
| Tel | (0)20 7610 3055 • (0)20 7610 3062 • Fax: (0)20 7610 3430 |
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| Tel | +972 (0) 8 946 0948 • Fax: +972 (0) 8 946 0861 |
| URL | Www.cytolab.com •E-mail: info@cytolab.com |
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| 2211 Riverside Drive • Suite 403 • Ottawa • K1H 7X5 • Canada |
| Tel | (613) 739 0083 or (866) 848 2847 • Fax: (613) 739 5850 |
| E-mail | Peprotechcanada@on.aibn.com |
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| Juan Enriquez No. 128 • Atos 2/A • Colonia Flores Magon • 91900 • Veracruz, Ver, Mexico |
| Tel | +52 (229) 931 2355 • Fax: +52 (229) 931 2352 |
| E-mail | Pepromex@prodigy.net.mx |
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| PeproTech Brazil FUNPEC |
| Rua Hudson 655 • 14.024-000 Ribeiro Preto • SP • Brazil |
| Tel/Fax | +55 (16) 620 1251 |
| URL | www.funpecrp.com.br •E-mail: funpecrp@uol.com.br |
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