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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein (AP)

Anti -Fluorescein (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #F5200-15A
Applications
Suitable for use in Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:1,000 to 1:5,000, 1.0 ug of Fluorescein conjugated IgG in a standard capture using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilution determined by the researcher.
Label
Alkaline Phosphatase (Calf Intestine) (Molecular Weight: 140kD)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
Concentration~ 1.0 mg/ml
FormSupplied as a liquid in 0.05M tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 50% glycerol; pH 8.0, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivi
ImmunogenFluorescein conjugated to Goat IgG
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and Fluorescein conjugated IgG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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