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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein (HRP)

Anti -Fluorescein (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IP IH IF Gel
Catalog #F5200-26
ApplicationsSuitable for use in ELISA, Western Blot, Dot Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000
Western Blot: 1:2000-1:5000
Immunohistochemistry: 1:500-1:2000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
PurityPurified by immunoaf nity chromatography.
ImmunogenFluorescein conjugated to Goat IgG
SpecificityRecognizes Fluorescein. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti peroxidase, anti goat serum and fluorescein conjugated IgG. No reaction against anti-pepsin or anti-goat IgGF(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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