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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein (TRITC)

Anti -Fluorescein (TRITC)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
Catalog #F5200-30
ApplicationsSuitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
LabelTetramethylrhodamine isothiocyanate (TRITC, Mr ~444D)
Absorption Wavelength550nm
Emission Wavelength570nm
Fluorochrome/Protein Ratio2 moles TRITC per mole of goat IgG
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostGoat
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with TRITC.
PurityPurified by immunoaf nity chromatography.
ImmunogenFluorescein conjugated to Goat IgG
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum and fluorescein conjugated IgG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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