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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Fluorescein (Texas Red)

Anti -Fluorescein (Texas Red)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IF
Catalog #F5200-31A
ApplicationsSuitable for use in Immunomicroscopy, FLISA, Flow Cytometry and FACS. Other applications have not been tested.
Recommended DilutionImmunofluorescence Microscopy: 1:500-1:2500
Flow Cytometry: 1:2000-1:10,000
Optimal dilutions to be determined by the researcher.
LabelTexas Red Sulfonyl Chloride (Molecular Weight 625D)
Absorption Wavelength596nm
Emission Wavelength620nm
Fluorochrome/Protein Ratio3.5 moles Texas Red per mole of goat IgG
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG F(abí)2
HostGoat
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Conjugated to Texas Red. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography, pepsin digestion and chromatographic separation.
ImmunogenFluorescein conjugated to goat IgG
SpecificityRecognizes Fluorescein. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum and fluorescein conjugated BSA. No reaction was observed against anti-pepsin or anti-goat IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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