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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IF

Catalog #	F5200-31A
Applications	Suitable for use in Immunomicroscopy, FLISA, Flow Cytometry and FACS. Other applications have not been tested.
Recommended Dilution	Immunofluorescence Microscopy: 1:500-1:2500
	Flow Cytometry: 1:2000-1:10,000
	Optimal dilutions to be determined by the researcher.
Label	Texas Red Sulfonyl Chloride (Molecular Weight 625D)
Absorption Wavelength	596nm
Emission Wavelength	620nm
Fluorochrome/Protein Ratio	3.5 moles Texas Red per mole of goat IgG
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG F(ab’)2
Host	Goat
Concentration	~1mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Conjugated to Texas Red. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography, pepsin digestion and chromatographic separation.
Immunogen	Fluorescein conjugated to goat IgG
Specificity	Recognizes Fluorescein. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum and fluorescein conjugated BSA. No reaction was observed against anti-pepsin or anti-goat IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.