Applications
Suitable for use in Immunomicroscopy, FLISA, Flow Cytometry and FACS. Other applications have not been tested.
Recommended Dilution
Immunofluorescence Microscopy: 1:500-1:2500 Flow Cytometry: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher.
Label
Texas Red Sulfonyl Chloride (Molecular Weight 625D)
Absorption Wavelength
596nm
Emission Wavelength
620nm
Fluorochrome/Protein Ratio
3.5 moles Texas Red per mole of goat IgG
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Fluorescein conjugated to goat IgG
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red. Reconstitute with 1ml sterile ddH2O.
Purity
Purified by immunoaffinity chromatography, pepsin digestion and chromatographic separation.
Specificity
Recognizes Fluorescein. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum and fluorescein conjugated BSA. No reaction was observed against anti-pepsin or anti-goat IgG F(c).