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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein Isothiocyanate (FITC) (HRP)

Anti -Fluorescein Isothiocyanate (FITC) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Catalog #F5249-02
ApplicationsSuitable for use in Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Substitution Ratio(Estimated) HRP:IgG is >1.5
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration1-2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.02% Thimerosal. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates..
PurityPurified by immunoaffinity chromatography.
ImmunogenFluorescein Isothiocyanate (FITC).
SpecificityRecognizes Fluorescein. No crossreactivity with immunoglobulins of any species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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