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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Fluorescein Isothiocyanate (FITC) (AP)

Anti -Fluorescein Isothiocyanate (FITC) (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified IH
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Catalog #F5249-03A
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~0.5mg/ml
FormSupplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide. Labeled with Alkaline Phosphatase.
PurityPurified by immunoaffinity chromatography.
SpecificityRecognizes Fluorescein isothiocyanate (FITC).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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