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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein Isothiocyanate (FITC) (HRP)

Anti -Fluorescein Isothiocyanate (FITC) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH IF Gel
Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation
Catalog #F5250-10B
ApplicationsSuitable for use in ELISA, Western Blot, Dot and Slot Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsELISA: 1:4000-1:8000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypePolyclonal
IsotypeIgG
HostSheep
ConcentrationNot Determined
FormSupplied as a liquid in PBS, pH 7.4, 50% glycerol. No preservative added. Labeled with horseradish peroxidase (HRP).
PurityPurified by affinity chromatography.
ImmunogenFluorescein isothiocyanate (Isomer I).
SpecificityRecognizes fluorescein isothiocyanate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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