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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified E FC

Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation.
Catalog #	F5250-11A
Fusion Partners	Spleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Applications	ELISA: 1.25-2.25ug/ml
	Flow Cytometry
	Optimal working dilutions to be determined by researcher.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	3H2148
Host	Rat
Source	Mouse
Concentration	~1mg/ml
Form	Purified IgM - liquid in PBS with 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity	Purified by affinity chromatography.
Immunogen	FITC conjugated mouse immunoglobulin.
Specificity	This antibody binds to both free FITC and FITC conjugated to protein such as antibodies. The reagent may be useful for amplification of staining using FITC conjugated reagents.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.