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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein Isothiocyanate (FITC)

Anti -Fluorescein Isothiocyanate (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E FC
Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation.
Catalog #F5250-11A
Fusion Partners Spleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
ApplicationsSuitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended DilutionELISA: 1.25-2.25ug/ml
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No3H2148
HostRat
Concentration~1mg/ml
FormPurified IgM - liquid in PBS with 0.1% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenFITC conjugated mouse immunoglobulin.
SpecificityThis antibody binds to both free FITC and FITC conjugated to protein such as antibodies. The reagent may be useful for amplification of staining using FITC conjugated reagents.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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