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You are here:Home » Antibodies » Abs to Epitope Tags » Anti -Fluorescein Isothiocyanate (FITC)

Anti -Fluorescein Isothiocyanate (FITC)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Catalog #F5250-11E
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~5mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenFluorescein isothiocyanate conjugated to KLH.
SpecificityRecognizes FITC.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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