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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein Isothiocyanate (FITC) (HRP)

Anti -Fluorescein Isothiocyanate (FITC) (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.
Catalog #F5250-12C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone NoNI 239
HostMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile ddH2O. Dilute further in PBS, pH 7.2.
PurityPurified
ImmunogenHighly purified fluorescein isothiocyanate isomer 1.
SpecificityRecognizes fluorescein isothiocyanate.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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