Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH IF

Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation.
Catalog #	F5250-17
Applications	Suitable for use in the detection of FITC labeled antigens and antibodies, as a capture antibody for FITC labeled bioploymers, ELISA, and as a second enzyme for Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Hybridoma	Hybridization of SP2/0 myeloma cells with spleen cells from Balb/c mice.
Affinity Constant	1.5 x 10e-8
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	1.BB.75
Host	Mouse
Concentration	~2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide before the addition of glycerol to 40%.
Purity	Purified by Protein A affinity chromatography. >90% tested by Immunoelectrophoresis.
Immunogen	FITC-BSA conjugate.
Specificity	Recognizes free fluorescein and FITC-labeled protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.