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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Fluorescein Isothiocyanate (FITC)

Anti -Fluorescein Isothiocyanate (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IF
Fluorescein isothiocyanate (FITC) is a small uoresent molecule that is commonly conjugated to antibodies for immuno uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli cation.
Catalog #F5250-17
ApplicationsSuitable for use in the detection of FITC labeled antigens and antibodies, as a capture antibody for FITC labeled bioploymers, ELISA, and as a second enzyme for Immunohistochemistry. Other applications have not been tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
HybridomaHybridization of SP2/0 myeloma cells with spleen cells from Balb/c mice.
Affinity Constant1.5 x 10e-8
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No1.BB.75
HostMouse
Concentration~2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein A affinity chromatography. >90% tested by Immunoelectrophoresis.
ImmunogenFITC-BSA conjugate.
SpecificityRecognizes free fluorescein and FITC-labeled protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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