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You are here:Home » Molecular Biology » MB-Serum Proteins » Folate Binding Protein, Bovine

Folate Binding Protein, Bovine

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Specifications

Folate Binding Protein, has a high affinity for folic acid and for several reduced folic acid derivatives and mediates delivery of 5 methyltetrahydrofolate to the interior of cells. Membrane bound and soluble forms of a high affinity folate binding protein have been found in kidney, placenta, serum, milk, and in several cell lines. The 2 forms have similar binding characteristics for folates, are immunologically crossreactive, and, based upon limited amino acid sequence data, are nearly identical. There may be a precursor product relationship between the membrane and soluble forms, the membrane form having additional amino acid residues and greater molecular weight. The membrane form has been shown to mediate the transport of folate in cells grown in physiologic concentrations of folate. A function for the soluble form, which is found in serum, milk, and urine, has not been identified.
Catalog #F5750-03
Protein content~2mg/ml (indirectly using the measured activity in ug/ml and the binding capacity of 8ug folic acid/mg folate binding protein.)
Activity:
~14.8ug/ml (Folate binding)
ApplicationsSuitable for use in ELISA. Other applications not tested
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceBovine Milk
Purity95% (HPLC)
FormSupplied as a liquid in PBS. No preservative added.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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