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You are here:Home » Antibodies » Abs to Vitamins » Anti -Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated

Anti -Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rat Purified E IH
Catalog #F5800-11
ApplicationsSuitable for use in ELISA, Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:2000-5000
Immunohistochemistry: 1:500-1000. Folic Acid-immunoreactive fibers located in the ventro-lateral part of the pulvinar nucleus of monkey brain. Also, immunoreactive fibers containing Folic acid in the dorsal mesencephalic region of the monkey brain. Fibers located between the ventricul and the lateral geniculate nucleus of the thalamus.
Optimal dilutions to be determined by the researcher.
SpecificityUsing a conjugate Folic acid-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
Compound Cross-reactivity ratio (a)
Folic acid-BSA 1 Folinic acid-BSA 1/>900 Tetrahydrofolic acid-BSA 1/>2,000 Methotrexate-BSA 1/>50,000 Riboflavin-BSA 1/>50,000 BSA 1/>50,000
(a) Folic acid-BSA concentration/unconjugated or conjugated compound concentration at half displacement.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRat
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, pH 7.2. No preservative added.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic Folic Acid conjugated to BSA.
SpecificityRecognizes protein conjugated Folic Acid (Vitamin B9). See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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