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You are here:Home » Antibodies » Abs to Vitamins » Anti -Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated

Anti -Folic Acid (Vitamin B9, Pteroylglutamic acid), Conjugated

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Specifications

Clone Host Grade Applications
Polyclonal Rat Purified IH
Catalog #F5800-11
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1:500-1:1000.
Optimal dilutions to be determined by the researcher.
SpecificityUsing a conjugate Folic acid-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
Compound Cross-reactivity ratio (a) Folic acid-BSA 1 Folinic acid-BSA 1>900
Tetrahydrofolic acid-BSA 1:>2,000
Methotrexate-BSA 1:>50,000
Riboflavin-BSA 1:>50,000
(a) Folic acid-BSA concentration/unconjugated or conjugated compound concentration at half displacement.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRat
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 100ul sterile dH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic Folic Acid conjugated to BSA.
SpecificityRecognizes protein-conjugated Folic Acid (Vitamin B9). See additional crossreactivity data.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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