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You are here:Home » Cloning » Cloning-Vectors » FOPflash (mutant Tcf binding sites) (TK-luciferase reporter) Negative Control

FOPflash (mutant Tcf binding sites) (TK-luciferase reporter) Negative Control

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Specifications

Transfection grade T cell factor (Tcf) reporter plasmid containing two full and one incomplete copy of the Tcf binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type Tcf binding sites.
Catalog #F5950
Size 5.5kb
Applications Suitable for use in TCF activity assays.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PurityPurified by immunoaffinity chromatography.
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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