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You are here:Home » Antibodies » Abs to Golgi Proteins » Anti -FTCD/Golgi protein, internal (LCHC1, Formiminotransferase Cyclodeaminase, Formimidoyltransferase Cyclodeaminase)

Anti -FTCD/Golgi protein, internal (LCHC1, Formiminotransferase Cyclodeaminase,
Formimidoyltransferase Cyclodeaminase)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #F6001-01
Domain Information COG3404: Methenyl tetrahydrofolate cyclohydrolase [Amino acid transport and metabolism]; COG3643: Glutamate formiminotransferase [Amino acid transport and metabolism]
ApplicationsSuitable for use in ELISA and Western Blot . Other applications not tested.
Recommended DilutionELISA: 1:32,000
Western Blot: 0.03-0.1ug/ml. A 55-60kD band observed in Human Liver lysates (calculated MW of 58.9kD according to NP_006648 and NP_996848). A 55-60kD band observed in Human Liver lysates
Optimal dilutions to be determined by the researcher.
Domain Information COG3404: Methenyl tetrahydrofolate cyclohydrolase [Amino acid transport and metabolism]; COG3643: Glutamate formiminotransferase [Amino acid transport and metabolism]
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See F6001-01-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5 mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02%sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide, aaCLREQGRGKDQPGRL, from the internal region of human FTCD. NP_006648.1; NP_996848.1.
SpecificityRecognizes human FTCD. Species sequence homology: Mouse. This antibody is expected to recognize both reported isoforms.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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