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You are here:Home » Antibodies » Abs to Prostaglandins » Anti -FPRP (F2alpha Prostaglandin Receptor Regulatory Protein, CD315, EWI-F, CD9 Partner-1, CD9P-1) (Biotin)

Anti -FPRP (F2alpha Prostaglandin Receptor Regulatory Protein, CD315, EWI-F, CD9
Partner-1, CD9P-1) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B FC
FPRP (F2alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130kD type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2alpha receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa22-813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa22-832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.
Catalog #F6065-50
ApplicationsSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 3T3-L1 cells in a total reaction volume 200ul.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile Tris buffered saline, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile Tris buffered saline, 0.1% BSA.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived recombinant mouse FPRP extracellular domain (aa22-832).
SpecificityRecognizes mouse FPRP extracellular domain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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