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Anti -FPRP (F2alpha Prostaglandin Receptor Regulatory Protein, CD315, EWI-F, CD9 Partner-1, CD9P-1) (Biotin)
Anti -FPRP (F2alpha Prostaglandin Receptor Regulatory Protein, CD315, EWI-F, CD9
Partner-1, CD9P-1) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B FC |
|
| FPRP (F2alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130kD type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2alpha receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton. Mature mouse FPRP is 858 amino acids (aa) in length. Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa22-813) plus a Pro-Thr-Glu-Leu ER retention signal. The ECD of mouse FPRP (aa22-832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively. | | | Catalog # | F6065-50 | | Applications | Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. | | Recommended Dilution | Western Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and reducing conditions. | | Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 3T3-L1 cells in a total reaction volume 200ul. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile Tris buffered saline, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Mouse | | Concentration | ~0.05mg/ml | | Form | Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile Tris buffered saline, 0.1% BSA. | | Purity | Purified by affinity chromatography. | | Immunogen | NS0-derived recombinant mouse FPRP extracellular domain (aa22-832). | | Specificity | Recognizes mouse FPRP extracellular domain. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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