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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 1 (FZD1)

Anti -Frizzled 1 (FZD1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B FC
Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a sevenpass transmembrane domain and a variable-length C-terminal tail.
Catalog #F6401-01C
ApplicationsSuitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit is for recombinant mouse Frizzled-1 is ~5ng/lane and ~25ng/lane under non-reducing and reducing conditions, respectively.
Flow Cytometry: 0.1mg/ml; Add 10ul to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant mouse Frizzled-1 is ~10ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5A1
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to mouse Frizzled-1 expressed in NS0 cells
SpecificityRecognizes mouse Frizzled-1. Detects Frizzled-1 on differentiated embryoid bodies derived from the mouse embryonic cell line D3 and on human HEK293 cells by Flow Cytometry. In Direct ELISA, it shows ~30% crossreactivity with recombinant mouse Frizzled-9. No crossreactivity with recombinant mouse Frizzled-2, -3, -4, -6, -7, -8, recombinant human Frizzled-5. or recombinant human MFRP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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