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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 1 (FZD1) (Biotin)

Anti -Frizzled 1 (FZD1) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IC FC
Frizzled-1 belongs to the frizzled family of 7-transmembrane domain proteins that are receptors for Wnt proteins. Frizzled-1 is expressed in a wide variety of tissues and is involved in cell differentiation, embryonal development and tumorigenesis.
Catalog #F6401-01E
ApplicationsSuitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.1mg/ml; 10ul labels 1-2.5x10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ml of a 25mg/ml stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome.
Immunocytochemistry: 5-10ug/ml in 4% paraformaldehyde- fixed cells.
Western Blot: 1-2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
HybridomaFusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived recombinant mouse Frizzled-1 (rmFrizzled-1) extracellular domain.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No5A3
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from PBS, BSA. Labeled wih Biotin. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
Endotoxin< 0.1EU/ug (LAL)
ImmunogenNS0-derived recombinant mouse Frizzled 1 extracellular domain
SpecificityRecognizes mouse Frizzled-1. Shows no crossreactivity with recombinant mouse Frizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry.
Species CrossreactivityHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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