| Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway and are involved in a variety of developmental processes. Frizzled proteins each contain a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain and a variable-length C-terminal tail. |
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| Catalog # | F6401-01F |
| Applications | Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: Use at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~2ng/lane under non-reducing and reducing |
| conditions. In this format, this antibody shows approximately 5% crossreactivity with rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8 and less than 1% crossreactivity with rmFrizzled-6. |
| Immunohistochemistry: Detects Frizzled-1 in cells and tissues at 15ug/ml. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Mouse |
| Concentration | ~0.05mg/ml |
| Form | Supplied as a lyophilized powder in PBS, pH 7.2, with BSA. Conjugated to Biotin. Reconstitute with 1ml sterile Tris-buffered saline pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA. |
| Purity | Purified by Immunoaffinity chromatography. |
| Immunogen | NS0-derived recombinant, murine, Frizzled-1 extracellular Cysteine-rich Domain (CRD) |
| Specificity | Recognizes mouse Frizzled-1. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
