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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 1, NT (FZD1)

Anti -Frizzled 1, NT (FZD1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
The Wnt signaling pathway plays important roles in development, cell proliferation, and cell movement. Wnts are secreted signaling proteins that are thought to be mediated by the Frizzled family of seven transmembrane proteins. Frizzleds act as receptors for Wnt proteins and may also be involved in cell migration patterns. Signaling by Wnt-1 via Frizzled homologues activates the function of Dishevelled, which represses the activity of glycogensynthase kinase-3, which in turn promotes the elevation of intracellular beta-catenin levels and accumulation of b-catenin in nuclei. The beta-catenin interacts with members of the TCF (T-cell factor) transcription family and activates expression of Wnt target genes such as c-myc and cyclin D1, which triggers cell proliferation, oncogenic transformation, and apoptosis inhibition. A recent study has also shown that LRP1 (Low Density Lipoprotein Receptor-1) interacts with Frizzled-1 to downregulate canonical Wnt signaling activity.
Catalog #F6401-01G
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlsMeWo human lymph node malignant melanoma, A549 human lung carcinoma, A375 human skin malignant melanoma, EWS502 human Ewing’s sarcoma, NGP96 human neuroblastoma, Jurkat human T-cell lymphocyte leukemia and HEK293 human embryonic kidney lysates.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the N-terminus of human Frizzled-1
SpecificityRecognizes human Frizzled 1, NT protein at 63kD. Additional bands at ~45 and ~125kD may be observed in Western blots of some human cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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