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You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Frizzled 2 (FZD2)

Anti -Frizzled 2 (FZD2)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B
Frizzled-2 belongs to the Frizzled family of 7-pass transmembrane receptors for Wnt proteins. Frizzled-2 is most extensively studied for its role in cardiac development and neural crest differentiation.
Catalog #F6401-07
HybridomaFusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled-2 (rmFrizzled-2) extracellular domain.
ApplicationsSuitable for use in Western Blot and Direct ELISA. Other applications not tested.
Recommended DilutionWestern Blot: Use at 1-2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-2. Using a colorimetric detection system, the detection limit for rmFrizzled-2 is ~25ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.
Direct ELISA: Use at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse Frizzled-2. The detection limit for rmFrizzled-2 is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No9j125
FormSupplied as a liquid in PBS, pH 7.2, 5% trehalose
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived rmFrizzled-2 extracellular domain
SpecificityRecognizes mouse Frizzled-2. No crossreactivity was observed with rmFrizzled-1, -3, -4, -6, -7, -8 or rhFrizzled-5.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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