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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 2 (FZD2) (Biotin)

Anti -Frizzled 2 (FZD2) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
Frizzled-2 belongs to the Frizzled family of 7-pass transmembrane receptors for Wnt proteins. Frizzled-2 is most extensively studied for its role in cardiac development and neural crest differentiation.
Catalog #F6401-07B
ReconstitutionReconstitute with sterile Tris-buffered saline, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA.
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: Use at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-2. The detection limit for rmFrizzled 2 is ~10ng/lane and 2ng/lane under non-reducing
and reducing conditions. In this format, this antibody shows ~25% crossreactivity with rmFrizzled 1 and less than 5% crossreactivity with rmFrizzled 3, rmFrizzled 4, rmFrizzled 5, rmFrizzled 6, rmFrizzled 7 and rmFrizzled 8.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.05mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, BSA. Conjugated to Biotin.
PurityPurified by immunoaffinity chromatography
ImmunogenNS0-derived rmFrizzled-2 extracellular cysteine-rich domain (CRD)
SpecificityRecognizes mouse Frizzled 2, extracellular domain specific.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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