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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 2 (FZD2)

Anti -Frizzled 2 (FZD2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
Wnt-Frizzled signaling is important for early development, regulating cell fate, polarity, differentiation, and migration. The frizzled gene, originally identified in Drosophila, is involved in the development of tissue polarity. The Frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a C-terminal Ser/Thr-xxx-Val motif, and they function as receptors for Wnt. Human Frizzled-2 (FZD2) is expressed in adult heart and fetal brain, lung and kidney. FZD2 activation leads to the release of beta/gamma subunit complexes from heterotrimeric G-proteins (presumably Gao and Gat) to activate phospholipase C and other effectors to stimulate a mobilization of intracellular Ca++. This does not involve the activation of the canonical WNT-beta-catenin pathway. FZD2 can also signal via the G-protein Gt2, transducin, a G-protein prominent in photo-transduction in the eye, to cyclic GMP phosphodiesterase. The calculated molecular weight of human FZD2 is ~63kD (length = 565aa) or ~71kD (length = 641aa). The observed molecular weight of ~ 85kD may be due to post-translational modifications such as glycosylation, which is predicted in the amino acid sequence.
Catalog #F6401-09A
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionImmunoprecipitation: 7ug/reaction
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlsImmunoprecipitation: HepG2 cells
Western Blot: Human CaCO2, PC-3, SKOV3, SW480, HepG2 and HEK293 cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the N-terminal region of human Frizzled-2 (FZD2).
SpecificityRecognizes human FZD2. On Western blots, it identifies a band at ~85kD. Species Sequence Homology: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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