| Wnt-Frizzled signaling is important for early development, regulating cell fate, polarity, differentiation, and migration. The frizzled gene, originally identified in Drosophila, is involved in the development of tissue polarity. The Frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a C-terminal Ser/Thr-xxx-Val motif, and they function as receptors for Wnt. Human Frizzled-2 (FZD2) is expressed in adult heart and fetal brain, lung and kidney. FZD2 activation leads to the release of beta/gamma subunit complexes from heterotrimeric G-proteins (presumably Gao and Gat) to activate phospholipase C and other effectors to stimulate a mobilization of intracellular Ca++. This does not involve the activation of the canonical WNT-beta-catenin pathway. FZD2 can also signal via the G-protein Gt2, transducin, a G-protein prominent in photo-transduction in the eye, to cyclic GMP phosphodiesterase. The calculated molecular weight of human FZD2 is ~63kD (length = 565aa) or ~71kD (length = 641aa). The observed molecular weight of ~ 85kD may be due to post-translational modifications such as glycosylation, which is predicted in the amino acid sequence. |
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| Catalog # | F6401-09A |
| Applications | Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested. |
| Recommended Dilution | Immunoprecipitation: 7ug/reaction |
| Western Blot: 1-3ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Controls | Immunoprecipitation: HepG2 cells |
| Western Blot: Human CaCO2, PC-3, SKOV3, SW480, HepG2 and HEK293 cell lysates |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide derived from the N-terminal region of human Frizzled-2 (FZD2). |
| Specificity | Recognizes human FZD2. On Western blots, it identifies a band at ~85kD. Species Sequence Homology: mouse. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
