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You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Frizzled 3 (FZD3)

Anti -Frizzled 3 (FZD3)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Catalog #F6401-10
ApplicationsSuitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml.
Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit is ~2ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/ml in fixed cells or tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 5% trehalose, before the addition of 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rmFrizzled-3 extracellular Cysteine-Rich Domain (CRD)
SpecificityRecognizes mouse Frizzled-3 extracellular Cysteine-Rich Domain (CRD). Crossreactivity: <1% with rmFrizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7 and rmFrizzled-8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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