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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 3 (FZD3)

Anti -Frizzled 3 (FZD3)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH IC
Catalog #F6401-10
ApplicationsSuitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml.
Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit is ~2ng/lane under non-reducing and reducing conditions.
Immunohistochemistry: 5-15ug/ml in fixed cells or tissues.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 5% trehalose, before the addition of 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived rmFrizzled-3 extracellular Cysteine-Rich Domain (CRD)
SpecificityRecognizes mouse Frizzled-3 extracellular Cysteine-Rich Domain (CRD). Crossreactivity: <1% with rmFrizzled-1, rmFrizzled-4, rmFrizzled-6, rmFrizzled-7 and rmFrizzled-8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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