Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 3 (FZD3)

Anti -Frizzled 3 (FZD3)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B IH FC
Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins.
Catalog #F6401-11C
ApplicationsSuitable for use in ELISA (direct), Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DIlutionWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 5-10ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as a goat anti-rat IgG (FITC).
Immunohistochemistry: A biotin conjugate of this antibody (F6401-11D) was used at a concentration of 8ug/ml to detect Frizzled 3 in frozen mouse embryo sections.
Optimal dilutions to be determined by the researcher.
HybridomaFusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled 3 extracellular Cysteine-Rich Domain (CRD).
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9j126
HostRat
SourceMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
Endotoxin<0.1EU/ug (LAL)
ImmunogenRecombinant protein corresponding to the extracellular CRD domain of mouse Frizzled 3 expressed in NS0 cells.
SpecificityRecognizes mouse Frizzled 3. Does not crossreact with recombinant mouse Frizzled 1, 4, 7 or 8. By Flow Cytometry and Immunohistochemistry, detects mouse Frizzled 3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Also detects Frizzled 3 on human HEK 293 cells by Immunocytochemistry and Flow Cytometry.
Species CrossreactivityHuman (HEK 293 cells)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links