| Frizzled-3 is an integral membrane protein that belongs to the Frizzled family of G-protein coupled receptors. Frizzleds function as receptors for Wnt proteins. |
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| Catalog # | F6401-11C |
| Applications | Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. |
| Recommended DIlution | Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing and reducing conditions. |
| Flow Cytometry: 5-10ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume < 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as an IgG (FITC) goat anti-rat IgG. |
| Immunohistochemistry: A biotin conjugate of this antibody (F6401-11D) was used at a concentration of 8ug/ml to detect Frizzled 3 in frozen mouse embryo sections. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled 3 extracellular Cysteine-Rich Domain (CRD). |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 9j126 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Endotoxin | <0.1EU/ug (LAL) |
| Immunogen | Recombinant protein corresponding to the extracellular CRD domain of mouse Frizzled 3 expressed in NS0 cells. |
| Specificity | Recognizes mouse Frizzled 3. Does not crossreact with recombinant mouse Frizzled 1, 4, 7 or 8. By Flow Cytometry and Immunohistochemistry, detects mouse Frizzled 3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Also detects Frizzled 3 on human HEK 293 cells by Immunocytochemistry and Flow Cytometry. |
| Species Crossreactivity | Human (HEK 293 cells) |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
