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You are here:Home » Antibodies » Antibodies-Wnt Proteins » Anti -Frizzled 3 (FZD3) (Biotin)

Anti -Frizzled 3 (FZD3) (Biotin)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH IC FC
Frizzled 3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins.
Catalog #F6401-11D
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit for recombinant mouse Frizzled-3 is ~0.5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 0.2mg/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome.
Immunocytochemistry: 8ug/ml detects Frizzled 3 in human HEK 293 cells or frozen mouse embryos.
Optimal dilutions to be determined by the researcher.
HybridomaFusion of a mouse myeloma with B cells obtained from a rat immunized with purified, recombinant mouse Frizzled 3 extracellular Cysteine-Rich Domain (CRD) expressed in NS0 cells.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
Clone No9j126
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
Endotoxin<0.1EU/ug (LAL)
ImmunogenRecombinant protein corresponding to mouse Frizzled 3 extracellular Cystine Rich Domain, expressed in NS0 cells.
SpecificityRecognizes mouse Frizzled 3. Does not crossreact with recombinant mouse Frizzled 1, 4, 7 or 8. Detected Frizzled 3 by Flow Cytometry and Immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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