| Frizzled 3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins. |
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| Catalog # | F6401-11D |
| Applications | Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-2ug/ml. The detection limit is ~0.5ng/lane under non-reducing and reducing conditions. |
| Flow Cytometry: 0.2mg/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume < 200ul. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. |
| Immunocytochemistry: 8ug/ml detects Frizzled 3 in human HEK 293 cells or frozen mouse embryos. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant mouse Frizzled 3 extracellular Cysteine-Rich Domain (CRD). |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 9j126 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, 5mg BSA. Labeled with Biotin. Reconstitute in 1ml sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Endotoxin | <0.1EU/ug (LAL) |
| Immunogen | NS0-derived recombinant mouse Frizzled 3 extracellular CRD |
| Specificity | Recognizes mouse Frizzled 3. Does not crossreact with recombinant mouse Frizzled 1, 4, 7 or 8. Detected Frizzled 3 by Flow Cytometry and Immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Species Crossreactivity: human |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
