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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 3 (FZD3) (Biotin)

Anti -Frizzled 3 (FZD3) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH IC FC
Frizzled 3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins.
Catalog #F6401-11D
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml. The detection limit for recombinant mouse Frizzled-3 is ~0.5ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 0.2mg/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome.
Immunocytochemistry: 8ug/ml detects Frizzled 3 in human HEK 293 cells or frozen mouse embryos.
Optimal dilutions to be determined by the researcher.
HybridomaFusion of a mouse myeloma with B cells obtained from a rat immunized with purified, recombinant mouse Frizzled 3 extracellular Cysteine-Rich Domain (CRD) expressed in NS0 cells.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9j126
HostRat
SourceMouse
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
Endotoxin<0.1EU/ug (LAL)
ImmunogenRecombinant protein corresponding to mouse Frizzled 3 extracellular Cystine Rich Domain, expressed in NS0 cells.
SpecificityRecognizes mouse Frizzled 3. Does not crossreact with recombinant mouse Frizzled 1, 4, 7 or 8. Detected Frizzled 3 by Flow Cytometry and Immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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