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You are here:Home » Antibodies » Abs to Wnt Proteins » Anti -Frizzled 5 (FZD5)

Anti -Frizzled 5 (FZD5)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Frizzled 5 belongs to the Frizzled family of integral membrane proteins that are receptors for Wnt proteins. Frizzled receptors have seven transmembrane domains and a long N-terminal cysteine-rich domain that bind directly to the Wnt proteins.
Catalog #F6401-17
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. The detection limit for rhFrizzled 5 is ~0.5ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit for rhFrizzled 5 is ~25ng/lane and 1ng/lane under non-reducing and reducing conditions, respectively.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 5% trehalose, 40% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenNS0-derived, recombinant human Frizzled 5 extracellular Cysteine-Rich Domain (CRD)
SpecificityRecognizes human Frizzled 5. Less than 5% crossreactivity with rmFrizzled 1, rmFrizzled 2, rmFrizzled 3, rmFrizzled 6, rmFrizzled 7 and rmFrizzled 8.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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